Coding
cbtA

Part:BBa_K2588006

Designed by: Daniel Wedemeyer   Group: iGEM18_Hamburg   (2018-10-05)


Cell division inhibitor cbtA

CbtA is a cytoskeleton toxin expressed by E. coli. It is the third part in our growth inhibition module. We decided to use this component of the type IV toxin-antitoxin system, because it interacts with FtsZ and MreB. Both proteins are involved in the elongation of E. coli during cell division as well as for building of the F-ring, to divide the cell.

Usage and Biology

CbtA inhibits the polymerization of FtsZ and MreB by binding to them and therefore inhibits cell division.1

References

  1. Tan, Q., Awano, N. & Inouye, M. YeeV is an Escherichia coli toxin that inhibits cell division by targeting the cytoskeleton proteins, FtsZ and MreB. Mol. Microbiol. 79, 109–118 (2011).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None